Genetic Analysis and Mapping of Rice(Oryza sativa.L) sd-sl Mutant

It is the bright road of rice breeding to study about dwarf mutant.On the other hand,dwarf mutants in rice are crucial for elucidating regulating mechanisms for plant growth and development.In the present study,the japonica cultivar 9522 was mutagenized by 60Co γ-Ray and then a rice dwarf mutant sd-sl was found which was dwarf and had smallish,olled leaves.Genetic analysis indicated that the phenotype was controlled by a single recessive gene.To map sd-sl locus,a F2 population was constructed from the cross between the sd-sl(japonica) and guangluai(indica).Firstly,the sd-sl locus was roughly mapped by InDel marker,OS604,in rice chromosome 6.And then several polymorphic markers were developed for further fine-mapping.At last the sd-sl locus was mapped between the two InDel markers,XL6-6 and XL6-1.The region was delimited to about 118 kb.These results provide a basis for molecular cloning and functional analysis of sd-sl.