2004•PubMedRequires access

[The induced apoptosis of recombinant adenovirus carrying melittin gene for hepatocellular carcinoma cell].

Li Bai, Changquan Ling, Chen Zhang, Wei Gu, Shaoxiang Li, Xue-Qiang Huang, Yani Zhang, Chaoqin Yu

Abstract

OBJECTIVE: To observe the induced apoptosis of recombinant adenovirus carrying melittin gene (Ad-rAFP-Mel) for hepatocellular carcinoma cell line (BEL-7402). METHODS: The morphological observe, DNA electrophoresis, TUNEL and Flow cytometry assay were used to study the apoptosis of BEL-7042 cell line transfected by Ad-rAFP-Mel. RESULTS: The morphological changes and apoptosis of BEL-7402 transfected by Ad-rAFP-Mel were confirmed with microscopy and DNA electrophoresis, TUNEL, Flow cytometry assay. The DNA ladder could be demonstrated on DNA electrophoresis in Ad-rAFP-Mel group. The apoptosis rates of BEL-7402 cells in Ad-rAFP-Mel, Ad-rAFP, and control groups were (21.5+/-2.4)%, (10.5+/-4.4)% and (3.0+/-1.4)% respectively by TUNEL assay (F = 38.0, P < 0.05) and were (7.3+/-0.5)%, (3.9+/-0.1)% and (0.8+/-0.1)% respectively by flow cytometry assay (F = 415.1, P < 0.05). CONCLUSION: It seems that melittin inducing apoptosis might be one of the antitumor mechanisms.

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Key concepts: Apoptosis, Molecular biology, Transfection, TUNEL assay, Flow cytometry, Melittin, Recombinant DNA, Biology