Genetic diversity analysis of Phytophthora sojae by using RAPD

Random amplified polymorphic DNA markers were used to assess genetic diversity among 15 isolates of Phytophthora sojae and 8 isolates of seven other Phytophthora species.152 RAPD markers were obtained,which were amplified by 12 random declaimer primers,with 84.2% polymorphisms.Using cluster analysis,the genetic diversity among Phytophthora species was detected,and RAPD similarity coefficients ranged from 0.533 to(0.783.) P.sojae was far away from seven other Phytophthora species in genetic distances.Moreover,a special RAPD marker of P.sojae was amplified by primer OPB06.RAPD analysis also indicated that there existed genetic diversity within P.sojae isolates,with similarity coefficient between 0.855 and 0.967.There was no correlation between DNA polymorphism of P.sojae isolates and their geographical origin,but the relationship between virulence pattern and RAPD markers was presented in some isolates.