Effect of Tumor Cells on Inducing Human Endothelial Cells to form New Blood Vessels in Vitro and the Role of VEGF

Objective Objective To study the influence of tumor cells on inducing human vascular endothelial cells to form new blood vessels and the role of vascular endothelial growth factor(VEGF) by setting up a stable angiogenesis culture system on Matrigel. Futher to discuss the relationship between angiogenesis and VEGF and its mechanism. Methods Culture human umbilical vein endothelial cells(HUVECs),set up a stable angiogenesis culture system by Matrigel. Oral squamous cells carcinoma cells lines were cultured and passaged,the conditional medium of tumor cells were prepared. Experiment was divided into concentration effective and time effective groups. The various groups were observed by inverted phase contrast microscope,then fixed by 95% ice alcohol. The expression of CD34 and kinase domain region receptor(KDR) of endothelial were detected by immunocytochemical method. The experiment results were tested by calculating the number of tubules in microscopic obversation and computer image-analysis. Finally the data was analyzed by statistics. Results After treatment with the conditional medium of tumor cells,the number of tubules increased and in certain ranges showing a dose-dependent and time-dependent manner(P 0.01);with the number of tubules increased,the expression of KDR of vascuular endothelial cells increased and in certain range showing a dose-dependent and time-dependent manner(P 0.01). Conclusion Tumor cells can promote in vitro induced human vascular endothelial cells by Matrigel to form new blood vessels and upregulate KDR expression and in certain ranges showing a dose-dependent and time-dependent manner.