Zinc Finger Protein A20 Inhibited NF-κB Activation,CD_40 and TNF-α Expression in Rats Peritoneal Mesothelial Cells Induced by LPS

Objective:To investigate the role of zinc finger protein A20( A20) on inflammatory response induced by LPS in cultured Sprague Dawley( SD) rats peritoneal mesothelial cells( RPMCs).Methods:The RPMCs were acquired and randomized to control group,LPS group,transfection A20 plasmid( pGEM-T easy-A20) group and empty plasmid group.After transfect pGEM-T easy-A20 into RPMCs with liposome 24 hours,stimulate RPMCs with LPS and cells as well as cell supernatant were obtained in different time.The protein expression of A20 and IκBα( inhibitor of nuclear factor-κB-α) were determined by western blotting;the mRNA expression of CD 40 and TNF-α were determined by RT-PCR;the level of TNF-α protein in cell supernatant was assayed by ELISA.Results:Compared with LPS group and empty plasmid group respectively,the protein of IκBα in pGEM-T easy-A20 group had no marked degradation( P 0.05);in addition,the expression of CD 40 and TNF-α mRNA as well as the level of TNF-α in cell supernatant were all decreased obviously( P 0.05);but there were no significant difference when compared with control group( P 0.05).Conclusion:A20 may depress the activation of NF-κB( nuclear factor-κB) and inflammatory response in cultured RPMCs induced by LPS.