Mutation Breeding of Candida

[Objective] The aim was to increase the lipid content of candida through mutation breeding.[Method] With candida as original strain,the original strains were mutated by ultraviolet mutation,microwave mutation and compound mutation with ultraviolet and microwave mutation,resp.and the strains with bigger biomass and Absorbance were screened.[Result] Compared with the control,the biomass of strain treated by ultraviolet mutation and microwave mutation were all increased.During the screening of high yield strain of yeast producing lipid,the compound mutation with ultraviolet and microwave mutation was a more effective method and a high yield yeast strain of Candida Yeast-1 was obtained.The biomass of the strain was 43.3% higher than that of the original strain and the Absorbance of lipid of the strain was 32.1% higher than that of the original strain.Its fat content was detected by soxhlet extraction and the fat content was increased 17.2%.[Conclusion] The candida treated by ultraviolet mutation,microwave mutation and compound mutation with ultraviolet and microwave mutation all could increase its lipid producing rate.