Induction and in vitro Culture of Hairy Roots in Peanut(Arachis hypogaea L.)

[Objective] To optimize the induction conditions of hairy roots in peanut,so as to lay foundation for producing resveratrol and the propagation of root-knot nematode.[Method]Effects of different Agrobacterium rhizogenes strains,explants type,bacteria concentration,co-culture time,infection time and AS concertration on the induction of hairy roots in peanuts were studied.[Result] Agrobacterium rhizogenes ACCC10060 had higher induction rate of hairy roots than strains ATCC11325.Leaflet had higher induction rate of hairy roots than cotyledon,the optimal bacteria concentration was 0.2-0.4,the optimal co-culture time was 2 d,the best infection time was 10-15 min,the dose of 75 μmol/L acetosyringone(AS) could improve induction rate of hairy roots.Hairy roots could grow independently on MS medium without hormone,and presence of opines from hairy roots of peanut confirmed that Ri plasmid of Agrobacterium rhizogenes was integrated into the genome of peanut.Hairy roots grew faster in liquid culture than in solid culture,and the optimal medium was MS liquid medium without hormone.[Conclusion] Establishment of hairy roots culture system of peanut provided a foundation for the improvement of peanut transgenic technique,industrial production of resveratrol and propagation of sterile root-knot nematodes.