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2009•Journal of Northwest UniversityRequires access

A study on extraction methods of microbial DNA from soils

Yan Wang

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Abstract

Aim To report methods of DNA extraction from soil microorganism.Methods Preparing the common-used and effective methods of DNA extraction with the designed method of DNA extraction from cornfield microorganism.Results All the four methods can obtain DNA fragments that are longer than 22kb from the cornfield microorganism.However,the quality of extraction DNA are different to some extent.The extraction DNA without purification could be amplified,and the relevant fragments by PCR with 16S rDNA universal primer could be acquired.Conclusion The designed method of DNA extraction is easy operation,quickly extraction and the acquired DNA is high both in purity and quality.

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What this paper is about

Aim To report methods of DNA extraction from soil microorganism.Methods Preparing the common-used and effective methods of DNA extraction with the designed method of DNA extraction from cornfield microorganism.Results All the four methods can obtain DNA fragments that are longer than 22kb from the cornfield microorganism.However,the quality of extraction DNA are different to some extent.The extraction DNA without purification could be amplified,and the relevant fragments by PCR with 16S rDNA universal primer could be acquired.Conclusion The designed method of DNA extraction is easy operation,quickly extraction and the acquired DNA is high both in purity and quality.

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Available abstract

Aim To report methods of DNA extraction from soil microorganism.Methods Preparing the common-used and effective methods of DNA extraction with the designed method of DNA extraction from cornfield microorganism.Results All the four methods can obtain DNA fragments that are longer than 22kb from the cornfield microorganism.However,the quality of extraction DNA are different to some extent.The extraction DNA without purification could be amplified,and the relevant fragments by PCR with 16S rDNA universal primer could be acquired.Conclusion The designed method of DNA extraction is easy operation,quickly extraction and the acquired DNA is high both in purity and quality.

Key concepts: DNA extraction, Microorganism, DNA, Extraction (chemistry), Primer (cosmetics), Chromatography, Polymerase chain reaction, Biology

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