Enalapril suppresses renal fibrosis after release of unilateral ureteral obstruction in rats

Objective: To investigate the effects of Enalapril (E) on renal fibrosis of rat release of unilateral ureteral obstruction (RUUO) model. Methods: Rats were randomly divided into 2 groups: (1) Sham group (n=6); (2) unilateral ureteral obstruction (UUO) group (n=12). Three days after induction of UUO, release the obstruction, then the rats in UUO group were divided into the following groups: (1) Release group as model control (n=6); (2) Release of UUO treated with Enalapril (10mg/kg/d, intragastric administration, n=6). Both the sham group and model control were intragastricly administrated with 0.5% CM-CNa. Two weeks after administration, the renal interstitial injury scores, collagen I, collagen III and CTGF were assessed and compared. Result: The renal interstitial injury scores, mRNA expression level of collagen I and collagen III, protein and mRNA expression level of CTGF were increased in RUUO model compared with sham group (P0.01) and reduced in Enalapril treated group compared with RUUO model control group (P0.01). Conclusions: Enalapril can obviously reduce renal tubulointerstital fibrosis after release of unilateral ureteral obstruction in rats. The mechanism of Enalapril may be involved with the reduction of CTGF.