Expression of Bcl-2 and Bax in CA1 area of hippocampus of vascular dementia rats

Objective To observe the expression of Bcl-2 and Bax in CA1 area of hippocampus of vascular dementia rats.Methods One hundred mice were randomly divided into sham operation group(Sham group) and vascular dementia model group(VD group).Each group were divided randomly into 1,2,4,8 and 12 week time point groups(n =10).VD groups were established by blocking four vessels.Learning and memory abilities were detected by Morris water maze.Apoplotic cells were detected by TUNEL.The expression of Bcl-2 and Bax were detected by immunohistochemistry.Results(1) Compared to Sham group,apoptotic cells were increased at different time point of VD group in CA1 area of hippocampus of rats, there were statistical significance(P 0.05 or P 0.01).(2) The positive expression of Bcl-2 and Bax were raised at 1 week,peaked at 4 week,at 8 week began to decline,to 12 week still higher in VD group in CA1 area of hippocampus of rats, there were statistical significance(P 0.05 or P 0.01).(3) Correlation analysis showed that vascular dementia rat hippocampal CA1 region of apoptotic cells and Bcl-2 and Bax protein expression was positively correlated(r = 0.845,P = 0.000; r =0.866,P =0.000).Conclusion Apoptosis related protein Bcl-2 and Bax participated to regulate apoptosis together in hippocampal CA1 area of vascular dementia rats.