Inhibition Effects of Polypeptide Drug Huilixinkang on Myocardial Apoptosis of Chronic Heart Failure Rats

OBJECTIVE:To explore the effects of the polypeptide drug Huilixinkang(HLXK) on the myocardial apoptosis in rats with chronic heart failure(CHF).METHODS:Rats were randomly divided into normal control group,model group,positive control group(intragastric administration of losartan 6 mg·kg-1,once a day) and HLXK low-dose,medium-dose and high-dose groups(intraperitoneal injection 10,30,90 μg·kg-1,twice a day) with 8 rats in each group.The latter 5 groups were given intraperitoneal injection of doxorubicin hydrochloride every 2 days for consecutive 15 days to establish CHF model and received relevant medicine.After 10 weeks of treatment,LVESV and EF of rats were measured by echocardiography,and expression of Bcl-2 and Bax in myocardial tissue were determined with immunohistochemistry technique.RESULTS:Compared with normal control group,LVESV of model group increased significantly,and EF decreased significantly(P0.01),the heart function markedly reduced and the expression of Bcl-2 protein decreased and expression of Bax protein enhanced significantly(P0.01).Compared with model group,LVESV level and expression of Bax protein in positive control group and HLXK medium-dose and high-dose groups decreased significantly while EF level and expression of Bcl-2 protein increased significantly(P0.05 or P0.01).The expression of Bax protein in HLXK low-dose group decreased significantly,and the expression of Bcl-2 protein enhanced significantly(P0.05).CONCLUSION:HLXK can inhibit myocardial apoptosis by enhancing the protein expression of Bcl-2 and inhibiting the protein expression of Bax.