2009•PubMedRequires access
[Biological characteristics of a chimeric rabies virus expressing canine parvovirus VP2 protein].
Xuefeng Niu, Xiaohui Liu, Zhao-Jin Sun, He-He Shi, Jing Chen, Bido Jiang, Jingchen Sun, Xiaofeng Guo
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Abstract
To obtain a bivalence vaccine against canine rabies virus and canine parvovirus, a chimeric rabies virus expressing canine parvovirus VP2 protein was generated by the technique of reverse genetics. It was shown that the chimeric virus designated as HEP-Flury (VP2) grew well on BHK-21 cells and the VP2 gene could still be stably expressed after ten passages on BHK-21 cells. Experiments on the mice immunized with the chimeric virus HEP-Flury (VP2) demonstrated that specific antibodies against rabies virus and canine parvovirus were induced in immunized mice after vaccination with the live chimeric virus.