2013•Zhongguo kangfu lilun yu shijianRequires access
Effect of Quyushengxin Method on Transforming Growth Factor-β_1/Smads in Ventricular Remodeling of Rats after Acute Myocardial Infarction
Shuai Wang
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Abstract
Objective To explore the effect of Quyushengxin Method on transforming growth factor-β1/Smads in ventricular remodeling of rats after acute myocardial infarction.Methods 50 male Wistar rats were randomly divided into control group,sham operation group,myocardial infarction group,Chinese medicine group and western medicine group with 10 rats in each group.Chinese medicine group was administered 3 d before the operation once a day.The rats were sacrificed 7 d later.The peripheral blood bone marrow mesenchymal stem cells(BMSCs) was detected and the mRNA level of TGF-β1,Smad3 and Smad7 were observed with RT-PCR.Myocardial c-kit cell number and HE staining results were also observed.Results The expression of TGF-β1 mRNA and Smad3 mRNA were higher and the expression of Smad7 mRNA was lower in the model group than in the sham operation group(P0.05).The expression of TGF-β1 mRNA and Smad3 mRNA were lower and the expression of Smad7 mRNA was higher in the Chinese medicine group than in the model group(P0.05).And the c-kit cell number was more in both Chinese medicine group and western medicine group than in the model group.And there was no significant difference between Chinese medicine group and western medicine group(P0.05).Conclusion Quyushengxin Method can inhibit ventricular remodeling after myocardial infarction in rats.