Cloning,expression and preliminary identification of the TβRII-like extracellular fragment protein from Schistosoma japonicum

To study the SjTβRII-like gene from Schistosoma japonicum (S. japonicum), the coding region of SjTβRII-like was obtained by RACE and the extracellular fragment of SjTβRII-like gene was cloned into the prokaryotic expression vector pET28a (+) then expressed in E.coli by IPTG induction. The recombinant protein was detected by SDS-PAGE. SD rats were immunized with the purified recombinant protein in order to obtain the antisera and analyze its immunogenicity and antigenicity by Western blotting.It was found that the encoding sequence of the novel SjTβRII-like gene was 2283 bp (encoding 760 amino acids). Recombinant expression plasmid was successfully constructed, which could elicit specific antibodies efficiently in SD rats, indicating its immunogenicity and antigenicity. Results suggested that the extracelluar fragment of the novel SjTβRII-like gene of S. japonicum could be expressed in the prokaryotic system with immunological and antigenic activities efficiently.