Effect of TSG on H_2O_2 Induced NF-κB Expression in Human Umbilical Vein Endothelial Cells

Objective To study the protective effect of TSG on human umbilical vein endothelial cells(hUVECs) injury induced by H2O2 and explore the related mechanism of action.Methods HUVEC treated with TSG(0.1,1.0,10.0 μmol/L) for 4 hours then exposed to 200 μmol/L H2O2 for 24 hours.Cell viability was determined by electron microscope observation and MTT assay.Gene expression of NF-κB and IκB were measured by RT-PCR and Western-blot. Results Compared with the model group,the cell viability of hUVEC impaired by H2O2 can be protected by different concentrations of TSG,especially by 1 μmol/L TSG treated groups.The expression of NF-κB mRNA and protein remarkably decrease(P0.01),and there were no effect for the expression of IκB. Conclusion TSG have a protective effect on the hUVECs impairment induced by H2O2,and the potential mechanism of action may be associated with downregulating the expression of NF-κB and blocking NF-κB/IκB signal pathway.