Study on chromosome culture by transabdominal chorionic villus sampling via the guidance of B-mode ultrasound

Objective: To evaluate the feasibility and safety of prenatal diagnosis by transabdominal chorionic villus sampling(TA-CVS) via the guidance of B-mode ultrasound in the first trimester of gestation.To research the method of long time culture and chromosome preparation of villi in early pregnancy.Methods: Chorionic villus specimens were obtained from 312 gravidas accepting prenatal diagnosis.All the samples were cultured for karyotype analysis.Results: A method of efficient chromosome analysis and chorionic villus cultivation was valuable.312cases(96.15%) was successful besides 12 cases failed because of being polluted.Conclusions: TA-CVS appears safe and feasible and might to be offered in the prenatal diagnosis in the first trimester of gestation.