Effects of NGF on IL-1β and IL-4 in BALF in Guinea Pig Models of Asthma

Objective To explore the effects of nerve growth factor(NGF)on IL-1β AND IL-4 in bronchoalveolar lavage fluid(BALF)in guinea pig models of asthma.Methods Thirty guinea pigs were randomly allocated to three groups:normal group;asthma group;anti-NGF group.The asthmatic models were established by injection and inhalation of OVA,the anti-NGF models by injection of anti-NGF antibody peritoneally three hours before inhalation OVA,and the animals in the control group were given PBS by both injection and inhalation.At 24 hour after the last OVA challenge,BALF was collected.Inflammatory cells in BALF were counted;the levels of IL-1β and IL-4 were measured by enzyme-linked immunosorbent assay(ELISA).Results Compared with the control group and asthma group,the total numbers of inflammatory cells,the counts of eosinophils,macrophage and lymphocyte of BALF in anti-NGF group showed significant differences(P0.05).The levels of IL-1β and IL-4 of BALF in asthma group were significantly different from those in the control and anti-NGF groups(P0.05).Conclusion NGF can regulate the levels of IL-1β and IL-4 of BALF in asthma models of guinea pig,NGF may contribute to immune inflammation in asthma.