Vector Construction Expression and Purification of Prokaryotic Expression of PP2A Gene of Rice

The rice protein phosphatase-2A(PP-2A) gene was amplified by polymerase chain reaction(PCR) from cDNA of young panicle in Nipponbare and cloned into the pGEX-6P-1 to construct the recombinant expression vector pGEX-6p-1-PP-2A.The recombinant was identified by restriction endonuclease digestion and sequencing analysis.E.coli JM109 bearing the plasmid was induced with 0.4mol/L IPTG to express the target protein.The expressed GST-PP2A fusion protein was characterized by SDS-PAGE and purified through the GSTrap FF resin,which would be helpful for further research.