2006•Tianjin Yike Daxue xuebaoRequires access
Comparison of a PCR assay with an ELISA assay for the detection of Toxoplasma gondii
Liu Pei-mei
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Abstract
Objective:To establish a rapid PCR assay with high sensitivity and specificity for the detection of Toxoplasma gondii DNA.Methods:A 194 segment of B1 gene was taken as the mode to perform PCR,and the reaction condition was optimized.Moreover,the sensitivity and specificity were determined.In addition,the mice were injected intraperitoneally with 104 tachyzoites,and then the blood specimens and serum samples were collected to perform a PCR assay and an ELISA assay for the detection of Toxoplasma gondii DNA and IgM respectively,and their results were compared.Results:In this study,the detection threshold was 0.2pg Toxoplasma gondii DNA.Moreover,there was not any cross-reaction with the DNA from Human beings,mice,Plasmodium yoelii and Trichinella spiralis.Besides these,Toxoplasma gondii DNA in blood samples were identified by PCR on the second day after inoculation,and total number of positive specimens was 13(13/15),whereas the IgM in serum were detected by ELISA only on the sixth day after inoculation,and the total number of positive specimens was 2(2/15).Statistically,there was significant difference between the positive number of blood samples and that of serum specimens(P0.05).Conclusion:The PCR assay is a rapid method with high sensitivity and specificity,and it can be used as a diagnostic test at early stage of Toxoplasma gondii disease.