[Effects of NO3- stress and recovery on chlorophyll fluorescence parameters and Atpase activities of cucumber seedling leaves].
Fengjuan Yang, Xiufeng Wang, Min Wei, Xiurong Su, Tong Yan
Abstract
Fengjuan Yang, Xiufeng Wang, Min Wei, Xiurong Su, Tong Yan
Abstract
With water culture, this paper studied the effects of NO3- stress and recovery on the chlorophyll contents, chlorophyll fluorescence parameters, and ATPase activities of cucumber seedling leaves. The results showed that under stress for 7 days, the chlorophyll a, chlorophyll b, total chlorophyll and carotenoid contents, intrinsic fluorescence (Fo), and Mg-ATPase and Ca-ATPase activities were increased significantly when the NO3- concentration was high (168 mmol x L(-1)), while the intrinsic photochemical efficiency (Fv/Fm), potential activities (Fv/Fo) and quantum yield (phiPS II) of PSII were decreased with increasing NO3- concentration. After recovery for 7 days, the chlorophyll and carotenoid contents of all treatments with NO3- were lower than CK, while the intrinsic fluorescence, intrinsic photochemical efficiency and quantum yield of PS II were similar to CK when the NO3- concentration was < or = 84 mmol x L((-1), and the Fv/Fm and phiPS II were significantly lower than CK when the NO3-concentration was > 84 mmol x L(-1). The potential activity of PS II was similar to CK when the NO3- concentration was 42 and 126 mmol x L(-1), but significantly lower than CK in other treatments. The activities of Mg-ATPase and Ca-ATPase were decreased firstly but increased then when the NO3- concentration was > 84 mmol x L(-1).
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With water culture, this paper studied the effects of NO3- stress and recovery on the chlorophyll contents, chlorophyll fluorescence parameters, and ATPase activities of cucumber seedling leaves. The results showed that under stress for 7 days, the chlorophyll a, chlorophyll b, total chlorophyll and carotenoid contents, intrinsic fluorescence (Fo), and Mg-ATPase and Ca-ATPase activities were increased significantly when the NO3- concentration was high (168 mmol x L(-1)), while the intrinsic photochemical efficiency (Fv/Fm), potential activities (Fv/Fo) and quantum yield (phiPS II) of PSII were decreased with increasing NO3- concentration. After recovery for 7 days, the chlorophyll and carotenoid contents of all treatments with NO3- were lower than CK, while the intrinsic fluorescence, intrinsic photochemical efficiency and quantum yield of PS II were similar to CK when the NO3- concentration was < or = 84 mmol x L((-1), and the Fv/Fm and phiPS II were significantly lower than CK when the NO3-concentration was > 84 mmol x L(-1). The potential activity of PS II was similar to CK when the NO3- concentration was 42 and 126 mmol x L(-1), but significantly lower than CK in other treatments. The activities of Mg-ATPase and Ca-ATPase were decreased firstly but increased then when the NO3- concentration was > 84 mmol x L(-1).
Key concepts: Chlorophyll fluorescence, Seedling, Chlorophyll, ATPase, Carotenoid, Chlorophyll a, Chemistry, Quantum yield