Application of sugar addition by pH-feedback strategy on biosynthesis of succinic acid

A pH-feedback strategy was established by investigation of consumption ratios of glucose and Na2CO3,which was used for neutralization of acid during the succinic acid production stage by Escherichia coli.Application of the strategy could maintain glucose concentration at a constant level.Compared with the batch fermentation,it was favourable for succinic acid accumulation when pH-feedback fermentation was adopted and glucose concentration maintained at a low level.In the case of controlling glucose concentration at 10g/L by pH-feedback fermentation,contents of the succinic acid in final titer reached 57.6g/L and production efficiency was 1.15g/(L·h).