Study on Technique of One-step Seedling Culture for Shoot Tip of Potato

The quality apical buds of potato were selected as explants to study the techniques of shoot tip detoxification,vigorous tube-seedlings and one-step seedling culture for potato.The results showed that the less bacterium the explants carried,the lower the contamination rate and the higher the shoot tip survival rate was;adding 0.2~0.8 mg/L of CCC to the MS culture medium had multiple effects for virus-free potato tube-seedlings,which included promoting proliferation and vigorous growth of seedlings,enhancing proliferation coefficient and rooting after subculture;moreover,the tube-seedlings had short temporary planting period and vigorous growth after transplanting.Applying technique of one-step seedling culture to large scale production can simplify process of tissue culture,shorten cycle of seedling breeding,and reducing cost of production.