The Development of Dorsal Hair Follicle and Expression of Lef-1 in Postnatal Murine

To study the histological characteristics of murine hair follicle and the function of Lef-1 at different hair follicle growth stages,the expression and localization of Lef-1 were investigated.HE staining was used to observe the morphology of the murine hair follicle at each stage,Western blotting and immunohistochemistry were used to determine the expression level and localization of Lef-1 protein at different hair follicle cycle stages.The result suggested that the gowth of murine hair follicle showed cyclic variation.For each stage,murine hair follicle had stage-specific characteristics.The result of Western blotting showed that Lef-1 was expressed in murine skin at different growth stages,and the expression level in hair follicle early growth period was higher than that in hair follicle middle and late growth periods.The result of immunohistochemistry showed that Lef-1 was expressed in sebaceous gland,root sheath and dermal papilla of hair follicle at different growth stages in different degree.The results indicate that cyclic variation of hair follicle depend on the expression level of Lef-1.