Experimental Study of Different Perfusion Methods and Neuroprotection during Deep Hypothermic Circulatory Arrest

OBJECTIVE To evaluate the protective effect of different perfusion during deep hypothermic circulatory arrest(DHCA).METHODSFourty healthy baby pigs were randomly divided into four groups(10 pigs per group).Grouup A:simple DHCA,Group B:DHCA+retrograde cerebral perfusion(RCP),Group C:DHCA+unilateral selective antegrade cerebral perfusion(U-SACP),Group D:DHCA+bilateral selective antegrade cerebral perfusion(B-SACP).Measure oxygen enhancement ratio(OER)from blood which were taken in different window around DHCA.Neural apoptosis was identified by TUNEL in the brain.Comparely analysis the change of OER and the depression of neural apoptosis in the brain among four groups was performed.RESULTSOER levels were significantly difference at post DHCA 30 min(T2),60min(T3),90 min(T4)between group A and group B,C,D(P0.05).The number of neural apoptosic cells in group B,C,D were decreased significantly during DHCA(P0.05).The level of apoptosic cells in group B was much higher than that in group C and D(P0.05),while that in group C was the same as the group D(P0.05).CONCLUSIONDHCA+RCP and DHCA+SACP are more both effective methods than DHCA on cerebral protection while the latter is more suitable.U-SACP and B-SACP are better than DHCA+RCP,but there was no difference between group C and D.