Measurement of Main Sugar Content Before & After the Pretreatment of Lignocellulose

The measurement method of main sugar content including glucose and xylose by high-performance liquid chromatography with evaporative light scattering detection(HPLC-ELSD) before and after the treatment of lignocellulose was developed.The optimum technical conditions were obtained as follows:the experimental drift tube at a temperature of 90 ℃,mobile phase of acetonitrile:water=70:30,flow rate as 1 mL/min,nitrogen flow rate as 2.0 L/min,and a good separation of glucose and xylose in the hydrolysate.The results showed that the correlation coefficient of the determination was 0.999(between 0.0094～110 mg/mL for glucose and between 0.031～62.34 mg/mL for xylose).The method had high precision with RSD as 0.54 % for glucose and 0.6 6 % for xylose respectively.The method had the advantages including simple operation,rapid measurement,high accuracy and good repeatability.