The improved method of isolation,culture and identification for mouse hepatic stellate cells

Objective To introduce a high efficient,convenient method for isolation and culture of mouse hepatic stellate cells(HSCs).Methods The method for isolation of normal mouse HSC was established with collagenase in situ liver perfusion,and optiprep density gradient centrifugation.HSCs were cultured in DMEM medium with FBS.Desmin and α-SMA were examined for identification of the cells.Results The quantity of HSCs was above 2~105 cells per mouse.The purity and the viability of the cells were about 93~97 percent.Conclusion The result showed that this method of isolation for HSCs is more convenient and steadier;the relatively steady HSCs can be used in the biological study of primary culture of HSCs.