Effect of CD147 monoclonal antibody on activity of matrix metalloproteinase on differentiation of osteoclast in vitro

Objective:To establish the model of differentiation and maturation of osteoclast(Ocs) in vitro and to studies the effect of CD147 monoclonal antibody on matrix metalloproteinases-2,9 expressed in differentiation of osteoclast in vitro.Methods:OCs were induced by peripheral blood mononuclear cells(PBMCs)of human using medium with macrophage colony stimulating factor(M-CSF) and receptor activator of nuclear factor-kappaB ligand(RANKL).PBMCs were divided into 2 groups,antibody group(RANKL+M-CSF+CD147Ab) and control group(RANKL+M-CSF).The maturation of osteoclasts was observed by tartrate-resistant acid phosphatase(TRAP) staining and the function of osteoclasts was analyzed by bone resorption test.The mRNA levels of CD147,MMP-2 and MMP-9 were detected by Real-Time PCR.And the activity of MMP-2,MMP-9 expressed by osteoclast was assessed by zymography analyses.Results:①The formation of osteoclast-like cells and bone resorption can be detected by TRAP staining and bone resorption test in control group,while the formation and activity of OCs were inhibited in antibody group.②The mRNA levels of CD147,MMP-2 and MMP-9 of the antibody group at 24 h,48 h time points were lower than the corresponding control group(P0.05).Moreover,the mRNA levels of MMP-2,MMP-9 were significantly positively correlated to the mRNA levels of CD147.③The digestion capacity of MMP-2,MMP-9 zymogen and active enzyme of the antibody group at 24 h,48 h time points were lower than the corresponding control group′s(P0.05) by Gelatin zymography analyses.Conclusion:CD147 monoclonal antibody can inhibit the expression and activity of MMP-2 and MMP-9 in the differentiation and maturation of osteoclast.One of the possible mechanisms activation of OCs regulated by CD147 may be achieved by regulating the expression and activity of MMP-2 and MMP-9.