Determination of quertetin and kaempferol in the burgeon leaves and old leaves of Guangxi Kudingcha

OBJECTIVE The contents of quertetin and kaempferol in the burgeon leaves or old leaves of Guangxi Kudingcha were compared.METHODS The burgeon and old leaves of Guangxi Kudingcha were extracted with methanol,ethanol or water.Contents of quertetin and kaempferol from different extracted solution were determined by HPLC.RESULTS The contents of quertetin and kaempferol were 500~750μg/g and 500~1 000μg/g in the burgeon leaves;While they were 1 100~1 800μg/g and 80~160μg/g in old leaves.CONCLUSIONS The contents of the components in the different leaves have some difference.The contents of quertetin and kaempferol in old leaves extracted with methanol are higher more.The old leaves of Guangxi Kudingcha will be more valuable in use.