Changes in Chemical Compounds and Enzyme Activity in Redried Lamina of Flue-cured Tobacco during Aging

Contents of polyphenol(chlorogenic acid and rutin),lipid,starch and protein,and activities of relevant enzymes,including PPO,lipoxygenase,α-amylase,and proteinase,in redried lamina of flue-cured tobacco were examined during aging.Results showed that the contents of polyphenol,lipid,starch and protein decreased by 20.0%,15.0%,11.5% and 12.2%,respectively,during the aging of 18 months.After the aging of 18 months,no significant changes occurred in the contents of rutin,starch,protein,yet the lipid content changed significantly(P≤0.05),the content of total polyphenol and chlorogenic acid changed much significantly(P≤0.01).The activities of polyphenol oxidase,lipoxygenase,amylase and proteinase all decreased during the whole aging.After aging of 18 months,the activities of four enzymes had highly significant difference(P≤0.01) with those before aging.