Analysis of cell morphology and immunophenotype detection in 120 patients with myelodysplastic syndrome

[Objective] To evaluate morphology of the blood and bone marrow cells and surface antigen detection of stem/progenitor and bone marrow cells in the diagnosis and prognosis of patients with myelodysplastic syndrome(MDS).[Methods] The study included 120 MDS cases and 20 cases as control group,which included 13 iron-deficiency anemia cases and 7 thrombocytopenic purpura cases.Blood routine was determined by Sysmex-xe-2100 blood analyzer.Blood and bone marrow smears were dyed by Wright-Giemsa dyeing method.Immunophenotypes were analyzed by flow cytometric using BD FACSCalib.[Results] MDS patients' peripheral blood showed two-line or three-line reduce.Bone marrows' myeloproliferative degree was active and obvious active.Pathological hematopoietic was seen with erythroid and myeloid lines,respectively account for 61.7% and 95.0%.Stem/progenitor cells' expression of antigens(CD34,HLA-DR) and myeloid early antigens(CD33,CD13,CD117) were significantly higher,and gradually heightened with the aggravation of the illness.MDS-RA,RCMD and RAEB type all had significant difference from control group(P0.05).The expression of CD34 in MDS-RAEB-Ⅱ type had presented difference with MDS-RA(P0.05).Myeloid late antigen(CD15) decreased significantly in MDS-RAEB-Ⅱ type(P0.05). [Conclusion] Blood and bone marrow cell morphology combined with immunophenotype detection in MDS patients is helpful for early diagnosis,treatment and prognosis judgment of MDS.