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2005•Xibei Nong-Lin Keji Daxue xuebao. Ziran kexue banRequires access

Telomerase activity in mouse oocyte and embryos during early development

Dou Zhong-ying

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Abstract

AbstrcatThe telomerase activity in mouse oocyte and preimplantation embryo was examined using the polymerase chain reaction-based on telomeric repeat amplification protocol (TRAP).Telomerase activity was detected in mouse oocyte,morulae and blastocyst and not in 2-cells and 8-cells embryo.The quantitative comparison results showed,by ladder densitomitric methodology,that the total product generated (TPG) were 85.782,96.231,269.331 units in mouse oocyte,morulae and blastocyst respectively.However,the TPG of single cell of morulae and blastocyst were 4.00,3.45 units.So,the oocyte had the highest telomerase activity level about 25-fold of blastocyst.

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What this paper is about

AbstrcatThe telomerase activity in mouse oocyte and preimplantation embryo was examined using the polymerase chain reaction-based on telomeric repeat amplification protocol (TRAP).Telomerase activity was detected in mouse oocyte,morulae and blastocyst and not in 2-cells and 8-cells embryo.The quantitative comparison results showed,by ladder densitomitric methodology,that the total product generated (TPG) were 85.782,96.231,269.331 units in mouse oocyte,morulae and blastocyst respectively.However,the TPG of single cell of morulae and blastocyst were 4.00,3.45 units.So,the oocyte had the highest telomerase activity level about 25-fold of blastocyst.

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Available abstract

AbstrcatThe telomerase activity in mouse oocyte and preimplantation embryo was examined using the polymerase chain reaction-based on telomeric repeat amplification protocol (TRAP).Telomerase activity was detected in mouse oocyte,morulae and blastocyst and not in 2-cells and 8-cells embryo.The quantitative comparison results showed,by ladder densitomitric methodology,that the total product generated (TPG) were 85.782,96.231,269.331 units in mouse oocyte,morulae and blastocyst respectively.However,the TPG of single cell of morulae and blastocyst were 4.00,3.45 units.So,the oocyte had the highest telomerase activity level about 25-fold of blastocyst.

Key concepts: Blastocyst, Oocyte, Telomerase, Embryo, Andrology, Biology, Embryogenesis, Cell biology

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