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Effects of NaCl stress on lipid peroxidation and activity of antioxidant enzymes in Elaeagnus oxycarpa

Ailijiang Mamat, Qiman Yunus, Qin Gong

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Abstract

One-year-old seedlings of Elaeagnus oxycarpa Schlecht. were treated with different concentration of NaCl stress. The changes of the membrane permeability ,malondialdehyde content(MDA),superoxide dismutase(SOD) activity,catalase (CAT) activity and peroxidase(POD) activity of leaves were studied. The results showed that the changes of membrane permeability took on an increasing trend at different stress time under each salt stress treatment. However, membrane permeability was gradually increased with prolonging the NaCl stress time. The malondialdehyde content was increased all the time,but its increase was not so significant, which reveals that the level of membrane liquid peroxidation was low. SOD and CAT activity in leaves of E. oxycarpa Schlecht. under NaCl stress with different concentrations were generally increased, and reached their maximum values with the increase of NaCl concentration when the seedlings of E. oxycarpa Schlecht. were treated 30 days. When NaCl stress was continued in 20 d, POD activity showed increasing firstly and then decreasing with the increasing NaCl stress, and it was reduced with the prolonging of the stress time at the same treatment. The correlation analysis showed that there were significant correlations among the membrane permeability, MDA content and the activity of antioxidant enzymes in leaves of E. oxycarpa Schlecht , which implied that the membrane permeability had a close relation with MDA content and the activity of antioxidant enzymes.

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What this paper is about

One-year-old seedlings of Elaeagnus oxycarpa Schlecht. were treated with different concentration of NaCl stress. The changes of the membrane permeability ,malondialdehyde content(MDA),superoxide dismutase(SOD) activity,catalase (CAT) activity and peroxidase(POD) activity of leaves were studied. The results showed that the changes of membrane permeability took on an increasing trend at different stress time under each salt stress treatment. However, membrane permeability was gradually increased with prolonging the NaCl stress time. The malondialdehyde content was increased all the time,but its increase was not so significant, which reveals that the level of membrane liquid peroxidation was low. SOD and CAT activity in leaves of E. oxycarpa Schlecht. under NaCl stress with different concentrations were generally increased, and reached their maximum values with the increase of NaCl concentration when the seedlings of E. oxycarpa Schlecht. were treated 30 days. When NaCl stress was continued in 20 d, POD activity showed increasing firstly and then decreasing with the increasing NaCl stress, and it was reduced with the prolonging of the stress time at the same treatment. The correlation analysis showed that there were significant correlations among the membrane permeability, MDA content and the activity of antioxidant enzymes in leaves of E. oxycarpa Schlecht , which implied that the membrane permeability had a close relation with MDA content and the activity of antioxidant enzymes.

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Available abstract

One-year-old seedlings of Elaeagnus oxycarpa Schlecht. were treated with different concentration of NaCl stress. The changes of the membrane permeability ,malondialdehyde content(MDA),superoxide dismutase(SOD) activity,catalase (CAT) activity and peroxidase(POD) activity of leaves were studied. The results showed that the changes of membrane permeability took on an increasing trend at different stress time under each salt stress treatment. However, membrane permeability was gradually increased with prolonging the NaCl stress time. The malondialdehyde content was increased all the time,but its increase was not so significant, which reveals that the level of membrane liquid peroxidation was low. SOD and CAT activity in leaves of E. oxycarpa Schlecht. under NaCl stress with different concentrations were generally increased, and reached their maximum values with the increase of NaCl concentration when the seedlings of E. oxycarpa Schlecht. were treated 30 days. When NaCl stress was continued in 20 d, POD activity showed increasing firstly and then decreasing with the increasing NaCl stress, and it was reduced with the prolonging of the stress time at the same treatment. The correlation analysis showed that there were significant correlations among the membrane permeability, MDA content and the activity of antioxidant enzymes in leaves of E. oxycarpa Schlecht , which implied that the membrane permeability had a close relation with MDA content and the activity of antioxidant enzymes.

Key concepts: Malondialdehyde, Chemistry, Membrane permeability, Superoxide dismutase, Catalase, Lipid peroxidation, Point of delivery, Antioxidant

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Effects of NaCl stress on lipid peroxidation and activity of antioxidant enzymes in Elaeagnus oxycarpa — Research Paper | ScholarLens