Protective effects of tumor necrosis factor α antibody on liver ischemia reperfusion injury in rats

ObjectiveTo study the protective effects of tumor necrosis factor α(TNF-α) antibody on liver ischemia reperfusion injury in rats. Methods120 SD rats were randomly divided into three groups, the normal control group(Group A,n=40), ischemia group(Group B,n=40) and TNF-α antibody group(Group C,n=40). After 60 minutes ischemia and followed reperfusion, the animals were killed at 0, 1h, 3h, 6h, 12h, serum concentration of alanine aminotransferase(ALT), malondialdehyde(MDA) was meassured and liver histopathology was observed. ResultsAfter ischemia reperfusion, the serum concentration of ALT and MDA in Group B significantly increased (P0.01) ,and the hepatic congestion was obvious compared with Group A. Treatment of TNF-α antibody could significantly decrease serum concentration of ALT and MDA in Group C compared with Group B(P0.01), and relieve hepatic congestion. ConclusionTNF-α antibody can suppress the inflammatory reaction, and protect injury induced by hepatic ischemia reperfusion in rats.