Analysis of the bacterial type and drug resistance of 99 strains of Mycobacterium

Objective:To understand the bacteria type and drug resistance of Mycobacterium from 99 patients in Xianju County,so as to provide the basis and reference for clinical treatment.Methods: Mycobacterium strains isolated from 99 patients with pulmonary TB were identified by using gene chip method.Further,rpoB gene,katG gene and inhA gene promoter of 88 M.tuberculosis strains were detected.Results:Eighty-eight strains of Mycobacterium isolates were M.tuberculosis complex group,accounting for 88.9%.Eleven strains were non-tuberculosis Mycobacterium,accounting for 11.1%.In the 88 strains of M.tuberculosis complex group,four strains were resistant to rifampicin,four strains were resistant to isoniazid and 6 strains were resistant to both of rifampicin and isoniazid.The resistance rate reached 15.9%.Seventy-four strains were highly sensitive to both of rifampicin and isoniazid,accounting for 84.1%.Conclusion: M.tuberculosis was predominant in the Xianju county of Zhejiang province whereas non-tuberculosis Mycobacterium accounted for more than one-tenth.Mycobacterium complex group were seriously resistant to rifampicin and isoniazid and had become a major threat to the control and treatment of TB.Therefore,it is necessary to strengthen the monitoring,treatment and management of drug-resistance.