Clinical significance of detection of bcr/abl gene in chronic myeioid leukemia.

Objective:To investiate the clinical significance of the detection of bcr./abl gene in chronic myeloid leukemia(CML).Methods:Expression of bcr/abl gene was determined by fluorogenic probe quantitative RT-PCR(FQ-RT-PCR)in 56 cases of CML.Results:No bcr/abl gene was revcaled in the control group,while in 56 cases of CML group the positive rate was 87.5%(49/56)with an average bcr/abl gene expression of 3.0×10~7-7.9×10~7 copies/L.The results of Ph chromatosome analysis showed that 25 patients were Ph(+)/bcr(+),2 patients were Ph(-)/bcr(+),4patients were Ph(+)/hcr(-)and 1 patients were Ph(-)/bcr(-) respectively.In 8 patients,before ainterferon therapy,average bcr/abl gene expression was(2.60±0.9)×10~7 copies/L,while after a-interferon therapy,the average bcr/abl gene expression was(1.78±0.6)×10~7 copies/L.The difference between two groups was significant(P0.05=.Conciusion:The quantitative detection of bcr/abl gene expression and dynamic evolution can help to make diagnosis,determine therapeutic effect and prognosis for CML.