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2010•Journal of Nuclear Agricultural SciencesRequires access

TETRAPLOID INDUCTION BY COLCHICINE AND IDENTIFICATION IN Cymbidium INTERSPECIFIC HYBRIDS

Guangdong Wang

Open publisher page 3 citations

Abstract

Tetraploid was successfully induced from in vitro plantlets of diploid Cymbidium interspecific hybrids treated with different concentration colchicine. Rhizomes were treated with colchicine at concentration of 0. 02% ,0. 05% , 0. 10% and 0. 20% for 24,48 and 72h. The results indicated that the treatment of rhizomes in colchicine solution at 0. 10% for 48h was the best,and its induction rate was 36% . The chromosome number of induced plants was 2n = 4x = 80,while that of the control plants was 2n = 2x = 40. Compared with the normal diploid plants,tetraploids were characterized by thicker and stronger roots,stems and leaves. The color of leaves and base of stems were darker and the plants shown slower growth rate. These new materials could be used in breeding of orchid.

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Tetraploid was successfully induced from in vitro plantlets of diploid Cymbidium interspecific hybrids treated with different concentration colchicine. Rhizomes were treated with colchicine at concentration of 0. 02% ,0. 05% , 0. 10% and 0. 20% for 24,48 and 72h. The results indicated that the treatment of rhizomes in colchicine solution at 0. 10% for 48h was the best,and its induction rate was 36% . The chromosome number of induced plants was 2n = 4x = 80,while that of the control plants was 2n = 2x = 40. Compared with the normal diploid plants,tetraploids were characterized by thicker and stronger roots,stems and leaves. The color of leaves and base of stems were darker and the plants shown slower growth rate. These new materials could be used in breeding of orchid.

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Available abstract

Tetraploid was successfully induced from in vitro plantlets of diploid Cymbidium interspecific hybrids treated with different concentration colchicine. Rhizomes were treated with colchicine at concentration of 0. 02% ,0. 05% , 0. 10% and 0. 20% for 24,48 and 72h. The results indicated that the treatment of rhizomes in colchicine solution at 0. 10% for 48h was the best,and its induction rate was 36% . The chromosome number of induced plants was 2n = 4x = 80,while that of the control plants was 2n = 2x = 40. Compared with the normal diploid plants,tetraploids were characterized by thicker and stronger roots,stems and leaves. The color of leaves and base of stems were darker and the plants shown slower growth rate. These new materials could be used in breeding of orchid.

Key concepts: Cymbidium, Colchicine, Rhizome, Ploidy, Interspecific hybrids, Biology, Interspecific competition, Botany

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