A new method with glucose for quantitative analysis of endodontic microleakage

Objective To establish a new method for the quantitative analysis of endodontic microleakage. Methods Forty straight human maxillary anterior teeth were divided randomly into two groups: Test group (20 roots) were obturated by laterally condensed gutta - percha and AHplus; the control group were 10 positive control and 10 negative control. The coronal part of roots were immersed in 1mol/L glucose solution and apical part in 1ml distilled water. Glucose solution was forced under a 15cm pressure toward the apical part of the root. Leakage along the root filling was measured by the concentration of glucose in apical reservoir at 1,2,4,7,10,15,20,25,30days with GOD method. Results Leakage was high in the positive control and could not be detected in the negative control. Leakage in the test group was obviously lower than that of in the positive control. Conclusion The method is simple,sensitive and accurate. The clinical relevance and feasibility are good.