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2005•Chinese Journal of Infection ControlRequires access

The application of DNA microarray in detecting respiratory virus

Linfu Zhou

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Abstract

Objective To develop a method to detect respiratory virus and its subtypies by DNA microarray directly.Methods A series of respiratory virus associated oligonucleotides were synthesized and prepared the DNA microarray.Influenza virus sample in cultured cells was treated for reverse transcription,amplification,fluorescent dye labelling,hybridization and scanning.Results Influenza virus subtypies were detected correctly.Conclusion Influenza A_1 and B in the cell culture can be detected correctly by DNA microarray.

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What this paper is about

Objective To develop a method to detect respiratory virus and its subtypies by DNA microarray directly.Methods A series of respiratory virus associated oligonucleotides were synthesized and prepared the DNA microarray.Influenza virus sample in cultured cells was treated for reverse transcription,amplification,fluorescent dye labelling,hybridization and scanning.Results Influenza virus subtypies were detected correctly.Conclusion Influenza A_1 and B in the cell culture can be detected correctly by DNA microarray.

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Available abstract

Objective To develop a method to detect respiratory virus and its subtypies by DNA microarray directly.Methods A series of respiratory virus associated oligonucleotides were synthesized and prepared the DNA microarray.Influenza virus sample in cultured cells was treated for reverse transcription,amplification,fluorescent dye labelling,hybridization and scanning.Results Influenza virus subtypies were detected correctly.Conclusion Influenza A_1 and B in the cell culture can be detected correctly by DNA microarray.

Key concepts: Microarray, Virus, DNA microarray, Virology, Biology, DNA, Oligonucleotide, Influenza A virus

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