The effects of myocardium of osteopontin expression of ACEI and aldosterone receptor antagonist in rats with myocardial infarction

Objective To investigate the cardioprotection of fosinopril and eplerenone and their influence on the osteopontin( OPN) in myocardium of rats with myocardial infarction( MI).Methods Rats with myocardial infarction were divided into 4 groups:myocardial infarction group,fosinopril( F) group( 10 mg/d),eplerenone( E) group( 10mg/d) and F + E group( fosinopril 5 mg/d + eplerenone 5 mg/d).Rats without myocardial infarction served as controls.After treatment for 6 weeks,the hemodynamics and heart function were detected; while the myocardial fibrosis was evaluated by pathological examination.RT-PCR was employed to assess the mRNA expression of OPN in the non-infarction area.Results In the myocardial infarction group,the heart function was significantly compromised,with myocardial fibrosis; which were reversed by fosinopril and/or eplerenone.Meanwhile,the OPN expression was increased dramatically in MI,which was reversed by fosinopril and/or eplerenone.The combined treatment provide significantly better efficacy.Conclusion Fosinopril and eplerenone can exert synergistic effect to improve the heart function and compromise the myocardial fibrosis which may be attributed to the reduction in OPN expression in the heart of rats with MI.