ScholarLens Back to search
2005•Lishizhen Medicine and Materia Medica ResearchRequires access

Effect of Troglitazone on Mouse Glucose Transporter I(mGLUT1) Expression

Mingzhu Liu

Open publisher page 0 citations

Abstract

Objective:To understand the possibility of effect of troglitazone on mGLUT1 expression.Methods:PPARγ ,RXRα and mGLUT1 cDNA clone into pCMX and pGL3b expression vector.The recombinant DNA transfected into NIH 3T3 cells,treated trogiltazone and confirm the mGLUT1 activity and mRNA expression by the trogiltazone through PPARγ activation with luciferase assay and Northern blot.Results:Troglitazone activates the mGLUT1 activity and increase the mGLUT1 mRNA expression through PPARγ activation.Conclusion:Troglitazone binds to PPARγ and activates mGLUT1 expression.

About this research paper

What this paper is about

Objective:To understand the possibility of effect of troglitazone on mGLUT1 expression.Methods:PPARγ ,RXRα and mGLUT1 cDNA clone into pCMX and pGL3b expression vector.The recombinant DNA transfected into NIH 3T3 cells,treated trogiltazone and confirm the mGLUT1 activity and mRNA expression by the trogiltazone through PPARγ activation with luciferase assay and Northern blot.Results:Troglitazone activates the mGLUT1 activity and increase the mGLUT1 mRNA expression through PPARγ activation.Conclusion:Troglitazone binds to PPARγ and activates mGLUT1 expression.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Objective:To understand the possibility of effect of troglitazone on mGLUT1 expression.Methods:PPARγ ,RXRα and mGLUT1 cDNA clone into pCMX and pGL3b expression vector.The recombinant DNA transfected into NIH 3T3 cells,treated trogiltazone and confirm the mGLUT1 activity and mRNA expression by the trogiltazone through PPARγ activation with luciferase assay and Northern blot.Results:Troglitazone activates the mGLUT1 activity and increase the mGLUT1 mRNA expression through PPARγ activation.Conclusion:Troglitazone binds to PPARγ and activates mGLUT1 expression.

Key concepts: Troglitazone, Transfection, Molecular biology, Peroxisome proliferator-activated receptor, Luciferase, Messenger RNA, Western blot, Chemistry

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Effect of Troglitazone on Mouse Glucose Transporter I(mGLUT1) Expression — Research Paper | ScholarLens