2004•Di-san junyi daxue xuebaoRequires access
Construction and identification of human aquaporin 1 gene recombinant adenovirus
Yang Yan
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Abstract
Objective To construct the recombinant adenovirus of human aquaporin 1 (AQP1) gene. Methods The replication deficient recombinant adenovirus encoding hAQP 1(pAdEasy 1/ hAQP1) was constructed by the method of DNA recombination and homogenous recombination in bacteria. The ECV 304 cells were transfected by the recombinant adenovirus. The target gene was identified by polymerase chain reaction (PCR). The titer and the infection rate were determined using the green fluorescent protein (GFP) expression in the shuttle plasmid. Results Restriction endonuclease and PCR analyses confirmed that the recombinant adenovirus was successfully constructed. The infection rate was high. Conclusion The recombinant adenovirus pAdEasy 1/ hAQP1 has been successfully constructed by the method of homogenous recombination in bacteria.