Impact of Hygromycin on Arabidopsis Suspension Cells and Calli

The impact of Hygromycin B on Arabidopsis suspension cells and calli was studied.The results show that the growth of Arabidopsis thaliana cells in vitro was significantly inhibited with Hygromycin B,and different effects and characteristics of inhibition were presented among different culture stages,especially between liquid and solid culture.On suspension subculture,calli inducement and calli subculture,the lethal critical concentrations of Hygromycin B for Arabidopsis thaliana cells were 25,2.5,1.5 mg/L in turn.A long or short time of treatment with Hygromycin B was necessary to impact on Arabidopsis thaliana cells fully.The inhibition efficiency became stable in 3 days in liquid and in 5 days on solid after treatment with Hygromycin B,but a considerable change presented among different concentrations.Furthermore,the probable causes of the different inhibition efficiency between liquid and solid culture conditions were discussed,and some suggestions were made for the selection of hygromycin-resistance marker on transformation of Arabidopsis suspension cells.