Detection of Common Intestinal Pathogens Infection with Gene Chip Technology

Objective To establish a PCR-Gene chip technology for detecting common intestinal pathogens that causes the diarrhea and provide the theory instruction to the intestinal tract inflammation.Methods Biotin-labeled universal primers for amplification of bacterial 16S rDNA and 23S rDNA were used to amplify extracted genome DNA.Appraising the detect and authenticate effect through expanding the target gene,the hybrid and the hybrid result analysis,meanwhile 100 fecal samples from patients with diarrhea were detected and identified using this assay and bacilliculture results,to inspect the sensitivity and specificity of gene chip technology.Results Among 100 fecal samples,50(50%) samples were positive for the intestinal pathogens by the PCR-Gene chip method;nevertheless 47(47%) samples were positive for the intestinal pathogens by bacterial culture;and the results of gene chip method were consistent with bacterial culture in 42 samples and the coincidence rate was 79.25%.Conclusions The gene chip system we established has high flux and the high accuracy,which is a rapid and accurate method to detect the common intestinal pathogens,and it is also well suitable for the epidemiology investigation and study.