Optimization on ultrasonic-assisted extraction technology of sucrose esters from tobacco by response surface methodology

The ultrasonic-assisted extraction technology of sucrose esters( SE) from tobacco was studied.The qualitative and quantitative analysis was performed by gas chromatography-mass spectrometry( GCMS) method,and the effect of various factors including extraction solvent,temperature,time and solid liquid ratio on the total extract yield of SE was investigated by response surface methodology( RSM). The results showed that the optimal extraction conditions were as follows: the extraction solvent acetonitrile,the solid liquid ratio 1∶ 29,the temperature 43 ℃ and the extraction time 16 min. Under the optimized conditions,the total extract yield of SE was 5. 039( actual) or 5. 067( theoretical),the average recovery was99. 87%,7. 74 % more than that obtained under the optimized conditions of single facter tests. The determination results of 10 kinds of tobacco leaf showed that the kinds and content of SE in tobacco leaves were different with different origin,variety and level.