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2008•Chinese Journal of PharmaceuticalsRequires access

Determination of Chondroitin Sulfate by Spectrophotometry with Crystal Violet

Lirong Teng

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Abstract

A spectrophotometric method was established for the determination of chondroitin sulfate. Crystal violet had a characteristic absorption peak at 583nm in pH 5.0 acetic acid-sodium acetate buffer solution. The maximal absorbance value was decreased by the complex which was formed from crystal violet with chondroitin sulfate. The decrease in absorbance value was linear with chondroitin sulfate in the concentration range of 0.01 - 2.7μg/ml. The average recovery was 99.7%.

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What this paper is about

A spectrophotometric method was established for the determination of chondroitin sulfate. Crystal violet had a characteristic absorption peak at 583nm in pH 5.0 acetic acid-sodium acetate buffer solution. The maximal absorbance value was decreased by the complex which was formed from crystal violet with chondroitin sulfate. The decrease in absorbance value was linear with chondroitin sulfate in the concentration range of 0.01 - 2.7μg/ml. The average recovery was 99.7%.

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Available abstract

A spectrophotometric method was established for the determination of chondroitin sulfate. Crystal violet had a characteristic absorption peak at 583nm in pH 5.0 acetic acid-sodium acetate buffer solution. The maximal absorbance value was decreased by the complex which was formed from crystal violet with chondroitin sulfate. The decrease in absorbance value was linear with chondroitin sulfate in the concentration range of 0.01 - 2.7μg/ml. The average recovery was 99.7%.

Key concepts: Chemistry, Absorbance, Chondroitin sulfate, Crystal violet, Acetic acid, Sulfate, Chromatography, Spectrophotometry

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