2008•Science and Technology of Food IndustryRequires access
Research on extraction technology of protein isolate in corn germ
Tao Cheng
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Abstract
The albumin,globin,prolamin and alkali-soluble protein in corn germ were separated by the water,5%Na2SO3,70%C2H5OH and 0.4%NaOH with the sequential extraction method.The optimal technology conditions for extraction were established.The results were as follows:when extraction temperature 40℃,water 35mL,extractin time 40min,the extraction rate of albumin was 39.35%~41.17%;when extraction temperature 40℃,Na2SO3 30mL,extractin time 30min,the extraction rate of globin was 30.78%~32.67%;when extraction temperature 50℃,C2H5OH 40mL,extractin time 20min,the extraction rate of prolamin was 2.87%~2.95%;when extraction temperature 50℃,NaOH 30mL,extractin time 30min,the extraction rate of alkali-soluble protein was 14.79%~15.61%。