2002•Zhonghua chuanranbing zazhiRequires access

Effects of vitamin E on the expression of type I collagen, TGFβ_1 and TIMP_2 of hepatic stellate cells

Dedong Xiang

Abstract

Objective To investigate the influence of vitamin E on type Ⅰ collagen, messenger RNA expression of transforming growth factor β 1 (TGFβ 1) and tissue inhibitor of matrix metalloproteinases-2 (TIMP 2) in hepatic stellate cells (HSC). Methods HSC s were cultured in vitro, the effect of vitamin E on type Ⅰ collagen was observed by mean of immunohistochemistry; mRNA expression of TGFβ 1 and TIMP 2 were investigated with in situ hybridization. Results In experiment group, vitamin E markedly inhibited expression of type Ⅰ collagen of HSC as compared with control group, but could not suppress mRNA expression of TGFβ 1 and TIMP 2.Conclusions Anti-fibrotic effect of Vitamin E may be implemented through inhibiting the expression of type Ⅰ collagen.

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Key concepts: Hepatic stellate cell, In situ hybridization, Messenger RNA, Transforming growth factor, Type I collagen, Immunohistochemistry, Matrix metalloproteinase, Vitamin