Establishment of Modified Deep Infiltrating Endometriosis Model in Rats

Objective To explore the establishment of rat models of modified deep infiltrating endometriosis(DIE) in the peritoneum between the uterus and bladder by autologous transplantation and discuss the advantage and disadvantage of the models.Methods Modified DIE models were established by transplanting endometrium to peritoneum at the junction between the uterus and bladder in rats,at the same time we removed parametrial free fat during the operation.Moreover,we contrasted them with DIE models at Douglas' cul-de-sac and non-improved DIE models in the peritoneum between the uterus and bladder.After two weeks,the rats were given the second operation.The adhesion degree of the implants was quantitatively evaluated.At the same time we observed histomorphology and histochemistry of modified DIE lesions.Results Rat DIE models were established successfully in all 3 groups,and the mean volume of the lesions showed no significant difference(P 0.05).The adhesion scores in the DIE models at Douglas' cul-de-sac and non-modified DIE models at the junction between the uterus and bladder were 2.38±0.74 and 2.75±0.46 respectively.The adhesion score in the modified DIE models at the junction between the uterus and bladder was 1.00±0.50,which reduced significantly compared with the other two groups(P 0.05).It showed that endometrial structure of lesions was similar to eutopic endometrium.Conclusion DIE models at the junction between the uterus and bladder in rats established by modified method are suitable for study due to simple manipulation and less adhesion.