Expression of caspase-3 mRNA and Cyt c protein in PC12 cell induced by MPP~+ and the Effect of Cerebrospinal Fluid containing Zhenganxifeng decoction

Objective:To investigate the Expression of caspase -3 mRNA and Cyt c protein in PC12 cell induced by 1-methyl-4-phenylpyridinium ion ( MPP + ) and the Effect of Cerebrospinal Fluid containing Zhenganxifeng decoction. Methods:Apoptosis was induced by addition of MPP + into PC12 cell cultures. And different concentration of CSF -ZGXF were administrated 24 h before addition of MPP + into PC12 cell cutrure. caspase-3 mRNA mRNA expression was assayed by real-time PCR. Cyt c protein protein was assayed by ELISA. Results:Expression of caspase-3 mRNA and Cyt c protein in PC12 cell treated with MPP + for 24 h was up-regulated compared with the control group. Expression of caspase-3 mRNA and Cyt c protein in PC12 cell were down-regulated in CSF-ZGXF group compared with the MPP + group on concentration dependent. Conclusion:The results suggest that Zhenganxifeng decoction inhibit expression upregulation of caspase-3 mRNA and Cyt c protein induced by MPP + ,and could be used as an effective drug for Parkinson disease.