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2003•Zhōnghuá yàoxué zázhìRequires access

Effects of total coumarines from the fruits of Cnidium monnieri on the production of NO,IL-1 and IL-6 from osteoblast in neonatal rat calvaria cultures

Qiao Zhang

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Abstract

OBJECTIVE To investigate the effects of total coumarines from the fruits of Cnidium monnieri (TCFC) on the production of NO,IL 1 and IL 6 from osteoblasts in neonatal calvaria cultures.METHODS Osteoblastic cells were isolated from newborn rat calvaria through trypsin and collagenase digestion.The proliferation of osteoblasts was assayed by 3H TdR.Levels of NO,IL 1 and IL 6 in the supernatant of rat osteoblast were respectively determined by Griess agents,examing proliferation of thymic cells and bioassay B9 cells as target cells.RESULTS TCFC significantly inhibited the production of NO,IL 1 and IL 6 spontanously or induced by TNF,IL 1 and LPS from osteoblasts in neonatal calvaria cultures.CONCLUSION The antiosteoporotic effect of TCFC is related to the inhibitory effects on the production of NO,IL 1and IL 6 from osteoblast.

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What this paper is about

OBJECTIVE To investigate the effects of total coumarines from the fruits of Cnidium monnieri (TCFC) on the production of NO,IL 1 and IL 6 from osteoblasts in neonatal calvaria cultures.METHODS Osteoblastic cells were isolated from newborn rat calvaria through trypsin and collagenase digestion.The proliferation of osteoblasts was assayed by 3H TdR.Levels of NO,IL 1 and IL 6 in the supernatant of rat osteoblast were respectively determined by Griess agents,examing proliferation of thymic cells and bioassay B9 cells as target cells.RESULTS TCFC significantly inhibited the production of NO,IL 1 and IL 6 spontanously or induced by TNF,IL 1 and LPS from osteoblasts in neonatal calvaria cultures.CONCLUSION The antiosteoporotic effect of TCFC is related to the inhibitory effects on the production of NO,IL 1and IL 6 from osteoblast.

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Available abstract

OBJECTIVE To investigate the effects of total coumarines from the fruits of Cnidium monnieri (TCFC) on the production of NO,IL 1 and IL 6 from osteoblasts in neonatal calvaria cultures.METHODS Osteoblastic cells were isolated from newborn rat calvaria through trypsin and collagenase digestion.The proliferation of osteoblasts was assayed by 3H TdR.Levels of NO,IL 1 and IL 6 in the supernatant of rat osteoblast were respectively determined by Griess agents,examing proliferation of thymic cells and bioassay B9 cells as target cells.RESULTS TCFC significantly inhibited the production of NO,IL 1 and IL 6 spontanously or induced by TNF,IL 1 and LPS from osteoblasts in neonatal calvaria cultures.CONCLUSION The antiosteoporotic effect of TCFC is related to the inhibitory effects on the production of NO,IL 1and IL 6 from osteoblast.

Key concepts: Calvaria, Osteoblast, Collagenase, Endocrinology, Internal medicine, Chemistry, Griess test, Andrology

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Effects of total coumarines from the fruits of Cnidium monnieri on the production of NO,IL-1 and IL-6 from osteoblast in neonatal rat calvaria cultures — Research Paper | ScholarLens